Department of Biotechnology

Dr. Rasheeda Bashir

Dr. Rasheeda Bashir
Dr. Rasheeda Bashir

Tenured Associate Professor

Department of Biotechnology

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Experience      

Date

Title

 

Institute

 

January  2014-to date

Assistant Professor (TTS)

Department of Biotechnology, Lahore College for Women University, Lahore

August 2012 - August 2013

Assistant Professor (IPFP)

Department of Biotechnology, Lahore College for Women University, Lahore

June 2009- July 2010

Teacher Assistant

Department of Microbiology and Molecular Genetics, University of the Punjab, Lahore

Honor and Awards

  1. Ph.D Scholarship, University of the Punjab, Lahore, Pakistan (2005-2006)
  1. Receive best performance award in the field of education by frog-e- taleem gold Medal award 2013

Memberships

Pakistan Zoological Society

Graduate Students /Postdocs/Undergraduate
/Students/Honour Students

Ph.D Thesis Supervision

On Going

  1. Name of Student: Maria Rafique

Topic: Production and immunological evaluation of polyvalent vaccine of   avian influenza (AIV-H9) and fowl cholera (Pasterulla multocida)

  1. Name of Student: Khazeema Yousaf
  2. Topic: Genetic causes of Glaucoma in Pakistani population

MS Students Supervised

 

Year

Name

Title

2019

Sadaf Bashir

Association of MCP-1 rs1024611 polymorphism with type II diabetic foot ulcer patients in Lahore, Punjab

2019

Umera Kauser

The association of VEGF rs699947 polymorphism in type II diabetic patients with or without diabetic foot ulcer from Punjab

2019

Iman Fatima

Screening of genetic variants p.M98K of optineurin gene in primary open angle glaucoma patients from Punjab

2019

Zernish Shabbir

Genetic variants analysis of p.D658G OF WDR36 gene in patients affected with primary open angle glaucoma from

2018

Hira Ali

Frequency of STAT4 gene single nucleotide polymorphism rs7574865 contribution to systematic lupus erthmatous in Pakistani population

 

2018

Samina Maqbool

Association of superoxide dismutase2 gene polymorphism rs4880 and open angle glaucoma in Pakistani population

 

2018

Ramish Shafique

Frequency of D835 mutation in FLT3 gene of leukemia of Pakistani population

2018

Resham Saleem

Genetic polymorphism and PACG: The association of SOD2 SNP rs4880 with PACG in Pakistan

2018

Aimen Naveed

Analysis of DeltaF508 mutation of CFTR gene in chronic pancreatitis patients of Pakistani population.

2018

Sana Aziz

Evaluation of antimicrobial activity of silver nanoparticles against wound infections

2017

Bushra Irfan

Association of Hepatocytes Growth factors gene polymorphism with primary angle closure glaucoma in Pakistani population

2017

Mehak Khalid

Genetic analysis of P-Type Adenosine Triphosphate Gene (ATP7B) in Pakistani Patients Affected with Wilson’s Disease

 

2017

Zunaira Tariq

The frequency of cationic trypsinogen (PRSS1) and serine protease inhibitor, kazal type 1 (SPINK1) gene mutations in Pakistani patients affected with chronic pancreatitis

 

2016

Harem Ali

Frequency of the p.G61E mutation in recessively inherited primary open glaucoma in Pakistani population.

2016

Rooha Tariq

Association of TP53 polymorphisms (rs1042522) and (rs17878362) with recessive primary open angle glaucoma in Pakistani population

2015

Rabia Mushtaq

Correlation between genotype and phenotype in adult primary open angle glaucoma in Pakistan with mutations in exon 3 of the MYOC gene

2015

Neelam Zaib

Antimicrobial susceptibility profiling and characterization of CTX-M gene in ESBL producing Klebsiella Pneumonia

2015

Sana Irum

Role of Myocillin (MYOC) gene sequence variation in juvenile open angel glaucoma (JOAG) in Pakistani population: Correlation between genotype and phenotype

2014

Khazeema Yousaf

Contribution of CYP1B1 gene mutations associated with primary congenital glaucoma in Pakistani population

2014

Hafsa Tahir

Screening of cytochrome p450 mutations associated with primary open angle glaucoma in Pakistani population

2013

Marukh Sanai

Genetic analysis of recessive glaucoma in Pakistan

BS/M.sc. Students Supervised

2019

Tayyaba Mahmood

In vitro mutagenicity assay of the cooking oils before and after and after heat treatment

2019

Resham razzaq

Assessment of mutagenic effects of artificial sweeteners by microsomal assay

2019

Nadia Tahir

Comparative mutagenic assessment of cooking oil and ghee before and after heat treatment by Salmonella typhimurium assay system

2019

Samra

Evaluation of mutagenicity of artificial sweeteners by Salmonella typhimurium assay

2018

Razia Gahni  

Gamma sterilization of surgical gauzes using cobalt-60 for elimination of residential microbial flora

2018

Noreen Mustafa

Use of gamma radiation to eliminate bioburden from syringes

2018

Iqra Shafique

A systematic study for the optimization and comparison of gamma irradiation doses with ethylene oxide sterilization on surgical gauzes

2018

Ammara Tahir

Dose dependent effect of gamma radiation on the selected eye ointments

2018

Amber Iftikhar

Effect of gamma radiation sterilization on eye ointment to eliminate the bioburden

2017

Afsheen Nasir

Isolation and identification of (Gram positive) from burn wounds and its antibiotic sensitivity

2017

Sana Saleem

Microbial flora (Gram negative) of burn wounds and  its antibiotic sensitivity

2017

Ameera Saleem

 

Effect of different physico-chemical factors on yogurt quality and production

2017

Andleeb Afzal

Effect of mix and pure culture of (lactic acid producing bacteria) on quality of local yogurt

2017

Nida Akram

Isolation and identification of lactic acid producing bacteria from different brands of yogurt

2016

Anum Asif

Effect of gamma radiation on fungal flora of rice (Basmati and Sella)

2016

Imrana Akram

Optimization of gamma dose to irradiate micro flora and insects from rice .To maximize rice safety and storability

2016

Rida Sadaqat

Dose optimization and assessment of proximate for the evaluation of nutrients and antioxidants of export quality irradiated basmati rice

2016

Fatima Anwar

Enhancement of shelf life and nutrient evaluation of locally grown export quality irradiated Sella rice

2015

Braira Wahid

 

Reducing the Risk of Nosocomial Illnesses by sterilization  of Nasal Oxygen Cannula Using Gamma Radiation (Cobalt – 60 Source)

2015

Resham Saleem

An  Assessment of Microbial Load on Nelaton Tube and its Effective Sterilization by gamma radiation

2015

Bakht Afroze

Targeting the Infection Causing Microbes on Tracheostomy Tube by the Optimizing the Dose of Gamma Radiation (CO-60).

2015

Hafiza Fizzah Zulfiqar

Estimation of microbial load and dose optimization for gamma irradiation of endotracheal tube

2015

Aneeqa javed

Targeting the viable microorganisms  on oxygen mask through optimized gamma radiation dose

2014

Maryam Qayyum

Reduction of microbial load of surgical forceps to minimal extent by gamma radiation

2014

Roha Tariq

Irradiation of cannula by cobalt – 60 source (gamma radiation) to ensure its safe usage by eliminating microbial load

2014

 

Tahira Haider

 Targeting the residing infection causing microbes using optimal dose of gamma radiation on feeding tubes (nasogastric tube)

2014

Faryal Akhtar

Optimization of  gamma radiation dose to minimize the microbial load on surgical scissors

2014

Mazia Amber

Identification and elimination of microbial load over commercially available medical drip set

Service Activity

Advisory and Administrative Services

Member Doctoral committee

Member research ethical review committee

Member Purchase committee (Chemicals)

Coordinator BS Program

BS Admission committee member

Member of synopsis defense committee (BS, MS, PhD.)

Brief Statement of Research Interest

  • Medical Genetics
  • Medical Microbiology
  • Immunology

Impact Factor Journals

  1. Rasheeda Bashir, Khazeema Yousaf, Hafsa Tahir, Marukh Sanai, Seema Qayyum, Shagufta Naz, Sadaf Naz. 2018. Clinical variability of CYP1B1 gene variants in Pakistani primary congenital glaucoma families. Pakistan journal of Medical Association. 68( 8) 1205-1211 (ISSN:0030-9982; Impact factor 0.616)

  2. Imtiaz A,Belyantseva IA, Beirl AJ, Fenollar-Ferrer C, Bashir R, Bukhari I, Bouzid A, Shaukat U, Azaiez H, Booth KT, Kahrizi K, Najmabadi H, Maqsood A Wilson EA, Fitzgerald TS, Tlili A, Olszewski R, Lund M, Chaudhry T, Rehman AU, Starost MF, Waryah AM, Hoa M, Dong L, Morell RJ, Smith RJH, Riazuddin S,Masmoudi S,Kindt KS, Naz S, Friedman TB.2018. CDC14A phosphatase is essential for hearing and male fertility in mouse and human. Human Molecular Genetics. 27 (5) 780-798 (ISSN: 0964-6906; Impact factor 5.340)

  3. Naz S, Imtiaz A, Mujtaba G, Maqsood A, Bashir R, Bukhari I, Khan MR, Ramzan M, Fatima A, Rehman AU, Iqbal M, Chaudhry T, Lund M, Brewer CC, Morell RJ, Friedman TB.2017. Genetic causes of moderate to severe hearing loss point to modifiers. Clinical Genetics. 91: 589-598 (ISSN: 0009-9163. Impact factor 3.326)

  1. Komal Najam, Imran Altaf, M Ashraf, M Adil Rasheed, Faiza Saleem, Neelma Munir, Rasheeda Bashir. 2017. In vitro evaluation of mutagenicity and genotoxicity of sitagliptin alone and in combination with artificial sweeteners. Tropical Journal of Pharmaceutical Research. 16 (8) 1841-1847 (ISSN: 1596-5996; Impact factor 0.569)

  1. Bashir R, Afroze B, Zulfiqar HF, Saleem R, Altaf I, Saleem F, Aslam F, Naz S. 2016. Microbiological Load of Ethylene Oxide Sterilized Medical Devices and its Elimination by Cobalt 60 Source. Journal of College for Physicians and Surgeon Pakistan. 26(6) 486-489 (ISSN: 1022-386X; Impact factor 0.554)

  1. Rizwana Raheel, Muhammad Ashraf, Aqeel Javeed, Rasheeda Bashir, Neelma Munir and Imran Altaf. Evaluation of antiviral and cytotoxic potential of ethanolic extract of Acacia nilotica against peste des petits ruminants virus in vitro cell culture. Indian Journal of Animal Sciences. 86 (11) 1274–1275 (ISSN 0367-8318; Impact Factor: 0.22)

  1. Ishrat Imran, Imran Altaf, Muhammad Ashraf, Aqeel Javeed, Neelma Munir, Rasheeda Bashir. 2016. In vitro evaluation of antiviral activity of leaf extracts of Azadirachta indica, Moringa oleifera, and Morus alba against the foot and mouth disease virus on BHK-21 cell line. Science Asia. 42: 392-396. (ISSN: 15131874; Impact factor 0.343)

  1. Rasheeda Bashir, Hafsa Tahir, Khazeema Yousaf Shagufta Naz , Sadaf Naz. 2015. Homozygous p.G61E mutation in a consanguineous Pakistani family with co-existence of juvenile-onset open angle glaucoma and primary congenital glaucoma. Gene. 570: 295–298 (ISSN: 0378-1119; Impact factor 2.415)

  2. Midhat Salman, Rasheeda Bashir, Ayesha Imtiaz, Azra Maqsood, Ghulam Mujtaba, Muddassar Iqbal & Sadaf Naz. Mutations of GJB2 encoding connexin 26 contribute to non-syndromic moderate and severe hearing loss in Pakistan. European Archive of Otorhinolaryngology 272 (8) 2071-2075 (ISSN: 0937-4477; Impact factor 1.660) 

  3. Faiza Saleem, Aisha Younas, Rasheeda Bashir, Shagufta Naz, Neelma Munir and Abdul Rauf Shakoori. 2014. Molecular Cloning and Characterization of Exochitinase A Gene of Indigenous Bacillus thuringiensis Pakistan Journal of Zoology, 46(6): 1491-1501. (ISSN: 0030-9923; Impact Factor: 0.400)

  4. Neelma Munir, Ayesha Saleha Iqbal, Imran Altaf, Rasheeda Bashir, Nadia Sharif , Faiza Saleem and Shagufta Naz. 2014. Evaluation of antioxidant and antimicrobial potential of two endangered plant species Atropa belladonna and Matricaria chamomilla. African Journal of traditional, complementary and alternative medicine. 11(5) 111-117 (ISSN 0189-6016; Impact factor 0.56)

  1. Rasheeda Bashir, Mahrukh Sanai, Adnan Azeem, Imran Altaf, Faiza Saleem , Sadaf Naz. 2014. Contribution of GLC3A locus to Primary Congenital Glaucoma in Pakistani population. Pakistan Journal of Medical Science. 30(6): 1341-1345 (ISSN: 1682-024X; Impact factor 0.166)

  1. Bashir R, Imtiaz Z A, Fatima A, Alam A, Naz S. 2013.The association of the c.42_52del11 mutation in TPRN with progressive or stable hearing loss. Biochemical Genetics. 51(5-6):350-357. (ISSN: 0006-2928; Impact factor 1.02)

  1. Khan R, Bashir R, Naz S. 2013. Mutations in SLC26A4 cause moderate to severe hearing loss in Pakistani families. Biochemical Genetics. 51: 514–523. (ISSN: 0006-2928; Impact factor 1.02)

  1. Rasheeda Bashir, Amara Fatima, Sadaf Naz. 2011. Prioritized sequencing of the second exon of MYO15A reveals a new mutation segregating in a Pakistani family with moderate to severe hearing loss. European Journal of Medical Genetics. 55(2) 99-102 (ISSN, 1769-7212; Impact factor 2.178)

  1. Rasheeda Bashir, Amara Fatima, Sadaf Naz. 2010. Mutations in CLDN14 are associated with different hearing threshold. The Journal of Human Genetics. 55(11) 767-670.(ISSN: 0340-6717 impact factor 2.570)

  2. R Bashir, A Fatima, S Naz. 2010. A deletion mutation in SANS results in atypical Usher syndrome. Clinical Genetics78(6)601-603.( ISSN‎: ‎0009-9163; Impact factor 3.128)

International publications in ISI Journals

1.Neelma Munir, Samra Pervaiz, Rukhama Haq, Rasheeda Bashir and Shagufta Naz. 2018. Effect of gamma radiation on post-harvest spoilage of spinach. International Journal Of Biology Pharmacy And Allied Sciences, 7(6)  991-998 (ISSN: 2277–4998; ISI Indexed)

2.Rasheeda Bashir, Neelam Zaib, Imran Altaf, Faiza Saleem, Qamar Sultana, and Shagufta Naz. 2016. Optimization of PCR for rapid detection of CTX-M gene in ESBL producing Klebsiella pneumoniae clinical isolates from Punjab, Pakistan. Malaysian journal of Microbiology,   12 (5) 365-369 (ISSN : 1823-8262; ISI indexed)               

3.Imran Altaf, Anika Khalid , Jawad Nazir , Waseem Shahzad ,Rasheeda Bashir. 2016. Outer membrane proteins and lipopolysaccharides mediated antibody response against bovine Pasteurella multocida type B-2. Malaysian journal of Microbiology. 12 (5): 370-375. (ISSN : 1823-8262; ISI indexed)

4.Neelma Munir, ayesha Saleha Iqbal, Imran Altaf, Rasheeda Bashir And  Shagufta Naz.2016. Phytochemical Analysis Of Extracts Of Atropa Belladonna And Matricaria Chamomila Obtained By Microwave Extraction Method. International Journal of Biology, Pharmacy and Allied Science. 5(1): 144-152. (ISSN: 2277–4998; ISI Indexed)     

5.Abdul Muqeet Khan , Imran Altaf , Shaguftasaeed , Mateen Abbas, , Asma Waris , Rukhama Haq , Rasheeda Bashir. 2016. Mycotoxin (Aflatoxin & Ochratoxin) And Pesticide Residues In Capsicum Frutescents In Punjab, Pakistan. International Journal of Biology, Pharmacy and Allied Sciences. 5(7): 1646-1654. (ISSN: 2277–4998;ISI Indexed)

6.Rasheeda Bashir, Braira Wahid, Aneeqa Javed, Imran Altaf, Neelma Munir, Rukhama Haq, Shagufta Naz. 2015. Reducing the risk of nosocomial illnesses by targeting the viable microflora on oxygen mask and nasal oxygen cannula through COBALT-60 SOURCE. International Journal of Biology, Pharmacy and Allied Sciences. 4(10): 6098-6106. ( ISSN: 2277–4998; ISI Indexed)

Books /Manuals Published

  1. A practical Manual of “Basic Technique in Molecular Biology “(2015) by Ch. Publisher Lahore, Pakistan.

  2. A Handbook of Microbiology” General Characteristic and Diagnosis of Pathologically Important Gram Positive Bacteria” (2015) by Khalid Publisher (Pvt) Limited, Urdu Bazaar, Lahore Pakistan.

  3. A Practical manual of “Basic of Cell Culture Techniques” (2014) by Awami Publishers (PVT) LTD Lahore Pakistan.

Proceeding Published

  1. Shagufta Naz, Rasheeda Bashir (2014) published proceeding on HEC funded one day workshop on “Emerging issues of cancers in Pakistan” Published by Ch. Publisher Anarkali Lahore, Pakistan.

  2. Naz A. Imtiaz, R. Bashir, G. Mubtaja, A. Maqsood, I. Bukhari, A. Rehman, R. J. Morell, T. B. Friedman. (2014). Alleles of the reported deafness genes are major contributors to the etiology of moderate to severe hearing loss in Pakistani population. Publication date, 2014/10/21. Conference, American Society of Human Genetics, Issue, 2946T

Research Grants and Contracts

  1. Research Grant as PI on HEC project No. NRPU/R&D/HEC/2738 entitled “Genetic Analysis of Disease Causing Genes for Glaucoma in Pakistani population” awarded by HEC; Award amount: 1.846 million. (2017-on going)

  2. Research Grant as PI of 0.5 Million from HEC (2013) Research project titled “Genetic Analysis of Glaucoma in Pakistan”. Awarded by HEC. Completed.

    Other Research or Creative Accomplishments

    Patents Filed

    “A process of Inoculum preparation to enhance the composting efficiency” submitted by Dr. Neelma Munir, Ms. Aisha Amin, Dr. Imran Altaf, Dr. Rasheeda Bashir, and Prof. Dr. Shagufta Naz in Gazette No. 192/2016

    “A process for identification and isolation of fourteen novel strains of Klebsiella pneumoniae subsp pneumonia with CTX-M gene from Pakistani population” by Dr. Rasheeda Bashir, Ms. Neelam Zeb, Prof. Dr. Shagufta Naz, Dr. Imran Altaf, Dr. Faiza Saleem, Dr. Neelma Munir, Dr. Farheen Aslam, Ms Rukhama Haq in Gazette No. 220/2015

    “Identification and isolation of two genetically heterogeneous novel Pakistani strains of klebsiella pneumonia subsp oxitoca BT2 and BT8 with CTX-M gene from Pakistani pneumonia patients" submitted by Dr. Rasheeda Bashir, Ms. Neelam Zeb, Prof. Dr. Shagufta Naz, Dr. Imran Altaf, Dr. Faiza Saleem, Dr. Neelma Munir, Dr. Farheen Aslam, Ms Rukhama Haq Published in Gazette No. 221/2015

     

    “Identification of two novel Pakistani strains Acinetobacter baumanii PK35 and Acinetobacter baumanii PK39 with new Delhi-Metallo –B- Lactamase-1 (BLANDM-1) gene isolated from Pakistani children’’ submitted by Dr. Farheen Aslam, Hira Lodi, Khwaja Ahmad Irfan Waheed, Prof. Dr. Shagufta Dr. Rasheeda Bashir. Published in Gazette No. 203/2015

    “Identification of three novel Pakistani strains Klebsiella pneumoniae PK1, Klebsiella pneumoniae PK9, with new Delhi-Metallo B- Lactamase-1 (BLANDM-1) gene isolated from Pakistani children, submitted by  Dr. Farheen Aslam, Hira Lodi, Khwaja Ahmad Irfan Waheed, Prof. Dr. Shagufta Dr. Rasheeda Bashir. Published in Gazette No. 204/2015

     

    Selected Professional Presentations

Oral Presentations

New trends in natural sciences II: public health ,food nutrition & safety24th  -26th  Oct, 2018        organized by Department of Biotechnology And Department of Zoology LCWU, Lahore, Pakistan

1st International Conference on Biotechnology Challenges and Opportunities in Pakistan. 11th -12th  October, 2017 organized by Lahore Garrison University

International Conference on “New Trends in Natural Sciences: from Basics to Applied. 25th  -27th  October, 2017 organized by Department of Biotechnology, And Department of Zoology LCWU, Lahore, Pakistan

3rd International Conference on Biotechnology: Challenges and Opportunities in Pakistan. 8th -9th  Feb, 2017            University of South Asia, Lahore, Pakistan

Research Colloquium. 16th -19th  Jan, 2017    DFDI, LCWU, Lahore, Pakistan

3rd  Regional Conference on Biosensors, Biodegradation, Biochips and Biotechnology, 20th -22th    April 2016            AIMST University Malaysia

13th  Medical Microbiology & Infectious Diseases Conference 25th -26th  March 2016 organized by Society of Pakistan Infectious Diseases Lahore, Pakistan

2nd  International Conference on Biotechnology : Challenges and Opportunities in Pakistan 24th -25th  February , 2016 organized by University of South Asia, Lahore, Pakistan

HEC funded one day workshop on “Emerging issues of cancers in Pakistan May 2014HEC Funded / Department of Biotechnology, LCWU, Lahore, Pakistan

Poster Presentations:

2nd International Conference on Biotechnology: Challenges and Opportunities in Pakistan. 24th-25th Feb, 2016 University of South Asia, Lahore, Pakistan

3rd International Conference on Biotechnology: Challenges and Opportunities in Pakistan. 8th -9th  Feb, 2017      organized by University of South Asia, Lahore, Pakistan