Department of Biotechnology

Farheen Aslam
Farheen Aslam

Assistant Professor

Department of Biotechnology

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Experience    

Assistant Professor at the Department of Biotechnology  22nd October 2012 to date.

Honor and Awards

  • Attended a “MALDI-TOF-MS Operator Training Course” ‘Proteomics’ with BrukerDaltonik GmbH Autoflex III TOF/TOF TM at Bremen, Germany from 19th through 21st of May, 2008.
  • Awarded with a Startup Research Grant on “Studying the effect on expression of a recombinant protein by changing the nucleotides at the promoter & start of the gene” of PKR 0.5 million by Higher Education Commission on June 4th, 2013.

Graduate Students /Postdocs/Undergraduate
/Students/Honour Students

Ms thesis supervised
  1. Characterization of Metallo Beta Lactamase gene blaIMP-1 isolated from different regions of Punjab (2013-2015)SyedaSaimaKanwal
  2. Characterization of Metallo Beta Lactamase gene blaVIM-1 isolated from blood samples of children from different regions of Punjab (2013-2015) AmnaSajjad
  3. Purification and characterization of Polymerase I from Geobacillus sp. (2013-2015) KainatWaheed
  4. Identification and characterization of antibiotic resistant New Delhi Metallo-β-lactamase-1 (blaNDM-1) gene, isolated from Pakistani children (2012-2014) HiraLodhi
  5. Cloning, expression and characterization of DNA polymerase I from Geobacillus sp. (2012-2014) Sumaira Akbar
  6. Cloning of keratinase gene in E.coli of Bacillus sp. Isolated from urban waste factory compost. TehreemYounas
  7. Expression Enhancement of Proinsulin Gene by Altering 5ʹ Untranslated and Translated Regions. (2011-2013) KunzaLatif
  8. Molecular detection and expression of azurin: an anti-cancer protein from local isolate of Pseudomonas sp. (2014-2016) HinaQaiser
  9. Construction and recombinant expression of anticancer exotoxin A (PE38KDEL) in Escherichia coli. (2014-2016) Adeen Farooq.
  10. MOLECULAR DETECTION OF GENETIC MUTATION IN POSSIBLE GENETIC ELEMENTS (CEBPA, NPM1) IN LEUKEMIA PATIENTS (2017-2019).  (sumreenaHussain).
BS thesis supervised
  1. Optimization of gamma radiation dose for reduction of microbial load on umbilical cord clamp. (2010-2014) Nadia Saeed
  2. Levels of bioburden on delivery kits before and after giving different doses of gamma radiation (2010-2014) Tahira Syed
  3. Effect of different doses of gamma radiation on reduction of microflora on applicators (2010-2014)Farwa Ashraf
  4. Effect of different doses of gamma radiation on reducing bioburden on needle holder (2010-2014)Hareem Ali
  5. Study on reducing the microbial content of eye ointment by different doses of gamma radiation (2010-2014)HamnaZubair
  6. Study on reducing flora of vingamungo by different doses of gamma radiations. (2012-2016). AimenNaveed
  7. Effect of different doses of gamma radiation on nutritional content of black grams (2012-2016) Maleeha kamran.
  8. Optimization of different doses of gamma radiation for reduction of microbial load from red kidney beans (2012-2016) Naila latif
  9. Proximate analysis of kidney beans after giving different doses of gamma radiation (2012-2016) Ridda Umer
  10. Identification of microbes in whitening creams and their elimination with different doses of gamma radiation (2011-2015)Hamna Afzal
  11. Detection and elimination of microbial load on drainage bags with different doses of gamma radiations (2011-2015)Inab Fatima
  12. Analysis of microbial load on blood transfusion set and dose optimization for reducing microflora by gamma radiations. (2011-2015)Khadija Riaz
  13. Detection and reduction of microbial load from different body lotions by optimizing doses of gamma radiation (2011-2015) Maryam Khan
  14. Estimation of microflora present in different brands of toothpastes and  reduction of micrflora by optimizing doses of gamma radiation (2011-2015) Maryam Mehmood
  15. Characterization of protease enzyme from milk. (2017-2019) AmnaAslam. Isolation and screening of proteases from cold climate microorganisms (2017-2019) NarjisKhatoon
  16. Isolation, Screening and Characterization of beta-galactosidase from local compost sample (2017-2019) LaibaRazzaq
  17. Study of beta-galactosidase isolated from milk (2017-2019) Amna Khan
  18. Characterization of protease enzyme from compost (2017-2019) Aqsa Mubeen.
  19. Isolation and characterization of microbes producing beta-galactosidase from soil samples (2017-2019) Ayesha Anwar.

Service Activity

  • Chemical purchase committee
  • Dengue Prevention and control committee
  • Doctoral committee of the department
  • Ph.D. and MS admission committee

Brief Statement of Research Interest

Cloning and expression of recombinant proteins, Characterization of commercially important enzymes, genetic profiling of leukemia, metabolomic analysis of hualongbing infected citrus varieties,

Publications

  • FarheenAslam, Qurra-tul  Ann Gardner, Hina Zain, Muhammad S  Nadeem, Muhammad Ali, Naeem Rashid and Muhammad Akhtar. Studies on the expression and processing of human proinsulin derivatives encoded by different DNA constructs. Biochim. Biophys. Acta, Proteins Proteomics. 2013 Jul;1834:2116-23. Impact factor 3.19 
  • Saleem M, Aslam F, Akhtar MS, Tariq M, Rajoka MI. Characterization of a thermostable and alkaline xylanase from Bacillus sp. and its bleaching impact on wheat straw pulp. World J MicrobiolBiotechnol. 2012 Feb;28(2):513-22. Impact factor 1.63 
  • Aslam, F., Younis, H., Gardner,Q.A., Rashid,N. and Akhtar,M.  Bostaurusproinsulin mRNA, partial cds. Accession no. JX041514. GenBank. 
  • Akhtar,M., Rashid,N., Younas,H., Gardner,Q.A. and Aslam,F. Bubalusbubalisbpi mRNA for proinsulin, partial cds. Accession no. AB234871. GenBank. 
  • Nadia Sharif, NeelmaMunir, FaizaSaleem, FarheenAslam and ShaguftaNaz. Prolific Anticancer Bioactivity of Algal Extracts (Review).American Journal of Drug Delivery and Therapeutics. 2014; 2349-7211: 060-072. 
  • Nadia Sharif, NeelmaMunir, FaizaSaleem, FarheenAslam&ShaguftaNaz. Mass cultivation of various algal species and their evaluation as a potential candidate for lipid production. Natural Product Research. 2015; http://dx.doi.org/10.1080/14786419.2015.1010163. Impact factor 1.009 
  • Amina Arif1, Naeem Rashid1*, Farheen Aslam2, Nasir Mahmood3 and Muhammad Akhtar. Biased expression, under the control of single promoter, of human interferon α-2b and Escherichia coli methionine amino peptidase genesin coli, irrespective of their distance from the promoter. Pak. J. Pharm. Sci., Vol.29, No.2, March 2016, pp.375-379. Impact factor 1.103 
  • Bashir R1, Afroze B1, Zulfiqar HF1, Saleem R1, Altaf I2, Saleem F1, Aslam F1, Naz S1. Microbiological Load of Ethylene Oxide Sterilized Medical Devices and its Elimination by Cobalt 60 Source.J Coll Physicians Surg Pak. 2016 Jun;26(6):486-9. doi: 2347. 0.4 
  • FarheenAslam, KunzaLatif, FaizaSaleem and ShaguftaNaz. A general insight into G-protein Coupled receptors. innov. Sci. 2(1); 71-74. 
  • HinaQaiser, FarheenAslam, SaimaIftikhar, Adeen Farooq. Construction and recombinant expression of Pseudomonas aeruginosatruncated exotoxin A in Escherichia coli. Cellular and Molecular Biology. 2018 | Volume 64 | Issue 1, 64-69.
Impact factor 1.37 
  • Khadija Gilani, ShaguftaNaz, FarheenAslam and William Gurley. A Comparison of Zinc, Phosphorous and Potassium Levels in Leaves and Fruit Pulp of Healthy and Huanglongbing Affected Citrus Cultivars. Journal of Plant Physiology & Pathology. 7:1. Impact factor 0.9.Vol. 18(27), pp. 664-669, July, 2019
  • FarheenAslam 1*, HooriaYounas 2, SaimaIftikharBajwa. Genomic sequencing and Recombinant Expression of Proinsulin isolated from Cow and Buffalo in Pakistan. 16 May 2019. African Journal of Biotechnology. 
  • GILANI K1, NAZ S1* AND FARHEEN ASLAM1 METABOLOMIC ANALYSIS OF CITRUS INFECTED WITH HUANGLONGBING. IJBPAS, October, 2019, 8(10): X-X.
  • Aslam F1*, Latif K1, Waseem R1, Naz S1 and Iftikhar S2. Recombinant Human Proinsulin Expression in E. Coli by Altering 5ʹ Untranslated and Translated Region. Gene Technology. 2019, 8:1.
  • Aslam F, Farooq A, Qaiser H, Bajwa S, Saleem F, et al. (2019) Molecular Detection and Expression of Azurin: An Anti-Cancer Protein from Local Isolates of Pseudomonas species. Gene Technol 7: 146. doi: 10.4172/2329-6682.1000146.
  • FarheenAslam 1*, HooriaYounas 2, SaimaIftikharBajwa. Genomic sequencing and Recombinant Expression of Proinsulin isolated from Cow and Buffalo in Pakistan. 16 May 2019. African Journal of Biotechnology.
  • KINZA NISAR1 , ROHEENA ABDULLAH1*, AFSHAN KALEEM1 , MEHWISH IQTEDAR1 , FAIZA SALEEM1 , TEHREEMA IFTIKHAR2 , FARHEEN ASLAM1Application of Plackett Burman and Box Behnken Design for the Optimization of β-glucosidase Production by Thermophillic Thermomyces dupontii. Revista de Chimie       Rev. Chim., 71 (9), 2020, 32-38.
  • Iftikhar S, Iqtedar M, Saeed H, Aftab M, Abdullah R, Kaleem A, Aslam F. Comparative and combinatorial study of Biogenic Bismuth nanoparticles with Silver Nanoparticles and Doxycycline against Multidrug Resistant Staphylococcus aureus BTCB02 and Salmonella typhi BTCB06. Revista Mexicana De Ingeniería Química. 2020 Oct 1;20(1):271-80.
  • Hassan, Faiza, Faisal Mehmood, Qamar uz Zaman, Dure Najaf Iqbal, Roeya Rehman, Farheen Aslam, Sadia Sagar, Nasir Masood, Arif Nazir, and Munawar Iqbal. "Evaluation of Physicochemical Properties and Metallic Contents in Vegetables Irrigated with Water from Different Sources." Polish Journal of Environmental Studies. Vol. 30, No. 2 (2021), 1-5
  • ud Din M, Ali A, Yasir M, Jilani MI, Shoaib S, Latif M, Ahmad A, Naz S, Aslam F, Iqbal M, Nazir A. Chemical Composition and in vitro Evaluation of Cytotoxicity, Antioxidant and Antimicrobial Activities of Essential Oil Extracted from Myristica Fragrans Houtt. Polish Journal of Environmental Studies. Pol. J. Environ. Stud. Vol. 30, No. 2 (2021), 1-6
  • Kaleem, Afshan, Rabail Javed, Kashmala Khalil, Mehwish Iqtedar, Roheena Abdullah, Lubna Riaz, Nadeem Ahmad, and Farheen Aslam. "Association between TSH status and prevalence of miscarriages and stillbirth." Bioscience Journal 37 (2021): e37022-e37022.
  • Abdullah, Roheena, Ammara Akhtar, Kinza Nisar, Afshan Kaleem, Mehwish Iqtedar, Tehreema Iftikhar, Faiza Saleem, and Farheen Aslam. "Process optimization for enhanced production of cellulases form locally isolated fungal strain by submerged fermentation." Bioscience Journal 37 (2021): e37021-e37021.
  • Iqtedar, Mehwish, Sidra Riaz, Saima Shehzaad Mirza, Mahwish Aftab, Afshan Kaleem, Roheena Abdullah, Ayesha Aihetasham, Farheen Aslam, and Myra Wasim. "Bioethanol production from urban cellulosic waste employing Alcaligenes faecalis HI-1 isolated from gut of termite Heterotermes indicola." Bioscience Journal 37 (2021): e37023-e37023.
  • Dilshad, Muhammad Akif, Shahid Sarwar, Farheen Aslam, Adnan Shabbir, and Shafqat Rasool. "Risk of Covid-19 infection during endoscopy: Efficacy of personal protective equipment (PPE) in protecting health professionals." Pakistan Journal of Medical Sciences 37, no. 4 (2021).
  • Iqtedar, Mehwish, Sidra Riaz, Saima Shehzaad Mirza, Mahwish Aftab, Afshan Kaleem, Roheena Abdullah, Ayesha Aihetasham, Farheen Aslam, and Myra Wasim. "Bioethanol production from urban cellulosic waste employing Alcaligenes faecalis HI-1 isolated from gut of termite Heterotermes indicola." Bioscience Journal 37 (2021): e37023-e37023.
  • Kaleem, Afshan, Rabail Javed, Kashmala Khalil, Mehwish Iqtedar, Roheena Abdullah, Lubna Riaz, Nadeem Ahmad, and Farheen Aslam. "Association between TSH status and prevalence of miscarriages and stillbirth." Bioscience Journal 37 (2021): e37022-e37022.
  • Abdullah, Roheena, Ammara Akhtar, Kinza Nisar, Afshan Kaleem, Mehwish Iqtedar, Tehreema Iftikhar, Faiza Saleem, and Farheen Aslam. "Process optimization for enhanced production of cellulases form locally isolated fungal strain by submerged fermentation." Bioscience Journal 37 (2021): e37021-e37021.
  • Yaqoob N, Munir H, Aslam F, et al. Antioxidant Potential and Phenolic Contents of Various Flaxseed Cultivars from Different Agro-Industrial Regions. Polish Journal of Environmental Studies. 2021;30(5):4325-4330. doi:10.15244/pjoes/132795.
  • Iftikhar, S., Aslam, F., & Akhtar, M. (2021). Serodiagnosis of Hepatitis B Virus Using PreS Antigen from Pakistani Isolate SBS001. Journal of Pharmaceutical Research International, 33(42A), 246-255. https://doi.org/10.9734/jpri/2021/v33i42A32411
  • Farheen Aslam*, Maryam Rasheeda and Saima Iftikhar BajwaB A REVIEW ON PROTEASES: THEIR SOURCES, TYPES AND APPLICATIONS. International Journal of Modern Pharmaceutical Research. IJMPR 2021, 5(6), 01-04

Research Grants and Contracts.

Awarded with a Startup Research Grant on “Studying the effect on expression of a recombinant protein by changing the nucleotides at the promoter & start of the gene” of PKR 0.5 million by Higher Education Commission on June 4th, 2013.

Selected Professional Presentations

  • Participated in an Orientation of OLK12 organized by Directorate of Faculty Development & Internationalization, LCWU 29th Sep-2nd Oct 2014
  • Participated in a National Conference on “Defeating the Public Enemy, The Mosquito: A Real Challenge (Poster Presentation) organized by Dengue Prevention and control Cell, LCWU, Lahore INSPIRE-Translational Education Partnership Programme. on 3-4 December, 2014
  • Participated in a National Workshop on “Controlling Biorisk in Lab” Organized by Department of Microbiology, University of Veterinary & Animal Sciences, Lahore and Bacteriology Department, Institute of Public Health, Lahore. May 11th-13th,
  • Participated in a two day workshop on “Biosafety and Biosecurity” Organized by Zoological Society, LCWU, Lahore Society for Promotion of Science and Technology, LCWU, Lahore.
  • Participated in Training of Presiding Officer and Senior Assistant Presiding Officers Local Government Election Commission of Pakistan. 13th Oct 2015 
  • Participated in International Conference on Climate change and Sustainable Development Organized by INSPIRE-Translational Education Partnership Program: Development of Collaborative Postgraduate Program on Climate Change. Held at Lahore College for Women University, Lahore. 28th-29th January 2016.
  • Organized a National Symposium on Biotechnology and Bioengineering: Application and Advantages. Department of Biotechnology, Lahore College for Women University, Lahore. 12th May 2016.